diff --git a/.github/workflows/unit-tests.yml b/.github/workflows/unit-tests.yml
index 0b6843c5a7033..9785b81ae9e0b 100644
--- a/.github/workflows/unit-tests.yml
+++ b/.github/workflows/unit-tests.yml
@@ -240,7 +240,7 @@ jobs:
           . ~/virtualenvs/pandas-dev/bin/activate
           python -m pip install --no-cache-dir -U pip wheel setuptools meson[ninja]==1.2.1 meson-python==0.13.1
           python -m pip install numpy --config-settings=setup-args="-Dallow-noblas=true"
-          python -m pip install --no-cache-dir versioneer[toml] cython python-dateutil pytz pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-asyncio>=0.17 hypothesis>=6.46.1
+          python -m pip install --no-cache-dir versioneer[toml] cython python-dateutil pytz pytest>=7.3.2 pytest-xdist>=2.2.0 hypothesis>=6.46.1
           python -m pip install --no-cache-dir --no-build-isolation -e .
           python -m pip list --no-cache-dir
           export PANDAS_CI=1
@@ -278,7 +278,7 @@ jobs:
           /opt/python/cp311-cp311/bin/python -m venv ~/virtualenvs/pandas-dev
           . ~/virtualenvs/pandas-dev/bin/activate
           python -m pip install --no-cache-dir -U pip wheel setuptools meson-python==0.13.1 meson[ninja]==1.2.1
-          python -m pip install --no-cache-dir versioneer[toml] cython numpy python-dateutil pytz pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-asyncio>=0.17 hypothesis>=6.46.1
+          python -m pip install --no-cache-dir versioneer[toml] cython numpy python-dateutil pytz pytest>=7.3.2 pytest-xdist>=2.2.0 hypothesis>=6.46.1
           python -m pip install --no-cache-dir --no-build-isolation -e .
           python -m pip list --no-cache-dir
 
@@ -351,7 +351,7 @@ jobs:
           python -m pip install --upgrade pip setuptools wheel meson[ninja]==1.2.1 meson-python==0.13.1
           python -m pip install --pre --extra-index-url https://pypi.anaconda.org/scientific-python-nightly-wheels/simple numpy
           python -m pip install versioneer[toml]
-          python -m pip install python-dateutil pytz tzdata cython hypothesis>=6.46.1 pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-cov pytest-asyncio>=0.17
+          python -m pip install python-dateutil pytz tzdata cython hypothesis>=6.46.1 pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-cov
           python -m pip install -ve . --no-build-isolation --no-index --no-deps
           python -m pip list
 
diff --git a/.github/workflows/wheels.yml b/.github/workflows/wheels.yml
index f8bfeaef94034..67c6f9bedd9c9 100644
--- a/.github/workflows/wheels.yml
+++ b/.github/workflows/wheels.yml
@@ -181,7 +181,7 @@ jobs:
         shell: pwsh
         run: |
           $TST_CMD = @"
-          python -m pip install hypothesis>=6.46.1 pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-asyncio>=0.17;
+          python -m pip install hypothesis>=6.46.1 pytest>=7.3.2 pytest-xdist>=2.2.0;
           python -m pip install `$(Get-Item pandas\wheelhouse\*.whl);
           python -c `'import pandas as pd; pd.test(extra_args=[\"`\"--no-strict-data-files`\"\", \"`\"-m not clipboard and not single_cpu and not slow and not network and not db`\"\"])`';
           "@
diff --git a/ci/deps/actions-310.yaml b/ci/deps/actions-310.yaml
index 200bfcffd9e61..414ce92552c70 100644
--- a/ci/deps/actions-310.yaml
+++ b/ci/deps/actions-310.yaml
@@ -14,7 +14,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - pytest-localserver>=0.7.1
   - boto3
 
diff --git a/ci/deps/actions-311-downstream_compat.yaml b/ci/deps/actions-311-downstream_compat.yaml
index 14644a4d786b7..6003728150d0e 100644
--- a/ci/deps/actions-311-downstream_compat.yaml
+++ b/ci/deps/actions-311-downstream_compat.yaml
@@ -15,7 +15,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - pytest-localserver>=0.7.1
   - boto3
 
diff --git a/ci/deps/actions-311-numpydev.yaml b/ci/deps/actions-311-numpydev.yaml
index c8da38bd5893f..b62e8630f2059 100644
--- a/ci/deps/actions-311-numpydev.yaml
+++ b/ci/deps/actions-311-numpydev.yaml
@@ -18,7 +18,6 @@ dependencies:
   # causes an InternalError within pytest
   - pytest-xdist>=2.2.0, <3
   - hypothesis>=6.46.1
-  - pytest-asyncio>=0.17.0
 
   # pandas dependencies
   - python-dateutil
diff --git a/ci/deps/actions-311-pyarrownightly.yaml b/ci/deps/actions-311-pyarrownightly.yaml
index c36a393087453..5455b9b84b034 100644
--- a/ci/deps/actions-311-pyarrownightly.yaml
+++ b/ci/deps/actions-311-pyarrownightly.yaml
@@ -15,7 +15,6 @@ dependencies:
   - pytest-cov
   - pytest-xdist>=2.2.0
   - hypothesis>=6.46.1
-  - pytest-asyncio>=0.17.0
 
   # required dependencies
   - python-dateutil
diff --git a/ci/deps/actions-311.yaml b/ci/deps/actions-311.yaml
index 609c864593614..66ba17509516f 100644
--- a/ci/deps/actions-311.yaml
+++ b/ci/deps/actions-311.yaml
@@ -14,7 +14,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - pytest-localserver>=0.7.1
   - boto3
 
diff --git a/ci/deps/actions-39-minimum_versions.yaml b/ci/deps/actions-39-minimum_versions.yaml
index 90433d177b523..8ea2a8a2f855a 100644
--- a/ci/deps/actions-39-minimum_versions.yaml
+++ b/ci/deps/actions-39-minimum_versions.yaml
@@ -16,7 +16,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - pytest-localserver>=0.7.1
   - boto3
 
diff --git a/ci/deps/actions-39.yaml b/ci/deps/actions-39.yaml
index 6b7467d252128..7ee3f655ea9ec 100644
--- a/ci/deps/actions-39.yaml
+++ b/ci/deps/actions-39.yaml
@@ -14,7 +14,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - pytest-localserver>=0.7.1
   - boto3
 
diff --git a/ci/deps/actions-pypy-39.yaml b/ci/deps/actions-pypy-39.yaml
index 1e3fead0b70a4..5a5a01f7aec72 100644
--- a/ci/deps/actions-pypy-39.yaml
+++ b/ci/deps/actions-pypy-39.yaml
@@ -16,7 +16,6 @@ dependencies:
   # test dependencies
   - pytest>=7.3.2
   - pytest-cov
-  - pytest-asyncio>=0.17.0
   - pytest-xdist>=2.2.0
   - hypothesis>=6.46.1
 
diff --git a/ci/deps/circle-310-arm64.yaml b/ci/deps/circle-310-arm64.yaml
index d5dfeaeda14b4..bc6354032798d 100644
--- a/ci/deps/circle-310-arm64.yaml
+++ b/ci/deps/circle-310-arm64.yaml
@@ -14,7 +14,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - pytest-localserver>=0.7.1
   - boto3
 
diff --git a/ci/meta.yaml b/ci/meta.yaml
index c02539e317b1a..aac5593e493b7 100644
--- a/ci/meta.yaml
+++ b/ci/meta.yaml
@@ -64,7 +64,6 @@ test:
   requires:
     - pip
     - pytest >=7.3.2
-    - pytest-asyncio >=0.17.0
     - pytest-xdist >=2.2.0
     - pytest-cov
     - hypothesis >=6.46.1
diff --git a/environment.yml b/environment.yml
index 1f8aeca0d197e..e41389c7f262a 100644
--- a/environment.yml
+++ b/environment.yml
@@ -16,7 +16,6 @@ dependencies:
   - pytest>=7.3.2
   - pytest-cov
   - pytest-xdist>=2.2.0
-  - pytest-asyncio>=0.17.0
   - coverage
 
   # required dependencies
diff --git a/pandas/tests/arrays/categorical/test_warnings.py b/pandas/tests/arrays/categorical/test_warnings.py
index c0623faccd325..68c59706a6c3b 100644
--- a/pandas/tests/arrays/categorical/test_warnings.py
+++ b/pandas/tests/arrays/categorical/test_warnings.py
@@ -1,19 +1,16 @@
 import pytest
 
-from pandas.util._test_decorators import async_mark
-
 import pandas._testing as tm
 
 
 class TestCategoricalWarnings:
-    @async_mark()
-    async def test_tab_complete_warning(self, ip):
+    def test_tab_complete_warning(self, ip):
         # https://github.com/pandas-dev/pandas/issues/16409
         pytest.importorskip("IPython", minversion="6.0.0")
         from IPython.core.completer import provisionalcompleter
 
         code = "import pandas as pd; c = pd.Categorical([])"
-        await ip.run_code(code)
+        ip.run_cell(code)
 
         # GH 31324 newer jedi version raises Deprecation warning;
         #  appears resolved 2021-02-02
diff --git a/pandas/tests/frame/test_api.py b/pandas/tests/frame/test_api.py
index 06bf169bf4dbc..5648cb15ef2b4 100644
--- a/pandas/tests/frame/test_api.py
+++ b/pandas/tests/frame/test_api.py
@@ -7,8 +7,6 @@
 
 from pandas._config.config import option_context
 
-from pandas.util._test_decorators import async_mark
-
 import pandas as pd
 from pandas import (
     DataFrame,
@@ -288,8 +286,7 @@ def _check_f(base, f):
         f = lambda x: x.rename({1: "foo"}, inplace=True)
         _check_f(d.copy(), f)
 
-    @async_mark()
-    async def test_tab_complete_warning(self, ip, frame_or_series):
+    def test_tab_complete_warning(self, ip, frame_or_series):
         # GH 16409
         pytest.importorskip("IPython", minversion="6.0.0")
         from IPython.core.completer import provisionalcompleter
@@ -299,8 +296,7 @@ async def test_tab_complete_warning(self, ip, frame_or_series):
         else:
             code = "from pandas import Series; obj = Series(dtype=object)"
 
-        await ip.run_code(code)
-
+        ip.run_cell(code)
         # GH 31324 newer jedi version raises Deprecation warning;
         #  appears resolved 2021-02-02
         with tm.assert_produces_warning(None, raise_on_extra_warnings=False):
diff --git a/pandas/tests/indexes/test_base.py b/pandas/tests/indexes/test_base.py
index 828700573c7ea..7620bf272db11 100644
--- a/pandas/tests/indexes/test_base.py
+++ b/pandas/tests/indexes/test_base.py
@@ -1243,14 +1243,13 @@ def test_cached_properties_not_settable(self):
         with pytest.raises(AttributeError, match="Can't set attribute"):
             index.is_unique = False
 
-    @td.async_mark()
-    async def test_tab_complete_warning(self, ip):
+    def test_tab_complete_warning(self, ip):
         # https://github.com/pandas-dev/pandas/issues/16409
         pytest.importorskip("IPython", minversion="6.0.0")
         from IPython.core.completer import provisionalcompleter
 
         code = "import pandas as pd; idx = pd.Index([1, 2])"
-        await ip.run_code(code)
+        ip.run_cell(code)
 
         # GH 31324 newer jedi version raises Deprecation warning;
         #  appears resolved 2021-02-02
diff --git a/pandas/tests/resample/test_resampler_grouper.py b/pandas/tests/resample/test_resampler_grouper.py
index b85ccdc70068f..0354cb9dfb3c3 100644
--- a/pandas/tests/resample/test_resampler_grouper.py
+++ b/pandas/tests/resample/test_resampler_grouper.py
@@ -4,7 +4,6 @@
 import pytest
 
 from pandas.compat import is_platform_windows
-from pandas.util._test_decorators import async_mark
 
 import pandas as pd
 from pandas import (
@@ -26,8 +25,7 @@ def test_frame():
     )
 
 
-@async_mark()
-async def test_tab_complete_ipython6_warning(ip):
+def test_tab_complete_ipython6_warning(ip):
     from IPython.core.completer import provisionalcompleter
 
     code = dedent(
@@ -37,7 +35,7 @@ async def test_tab_complete_ipython6_warning(ip):
     rs = s.resample("D")
     """
     )
-    await ip.run_code(code)
+    ip.run_cell(code)
 
     # GH 31324 newer jedi version raises Deprecation warning;
     #  appears resolved 2021-02-02
diff --git a/pandas/util/_test_decorators.py b/pandas/util/_test_decorators.py
index 0cc2a125c1e26..e71b4881a58a4 100644
--- a/pandas/util/_test_decorators.py
+++ b/pandas/util/_test_decorators.py
@@ -44,7 +44,6 @@ def test_foo():
     IS64,
     is_platform_windows,
 )
-from pandas.compat._optional import import_optional_dependency
 
 from pandas.core.computation.expressions import (
     NUMEXPR_INSTALLED,
@@ -204,16 +203,6 @@ def documented_fixture(fixture):
     return documented_fixture
 
 
-def async_mark():
-    try:
-        import_optional_dependency("pytest_asyncio")
-        async_mark = pytest.mark.asyncio
-    except ImportError:
-        async_mark = pytest.mark.skip(reason="Missing dependency pytest-asyncio")
-
-    return async_mark
-
-
 def mark_array_manager_not_yet_implemented(request) -> None:
     mark = pytest.mark.xfail(reason="Not yet implemented for ArrayManager")
     request.applymarker(mark)
diff --git a/pyproject.toml b/pyproject.toml
index 85bb937fe431f..7178c9407ce78 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -62,7 +62,7 @@ repository = 'https://github.com/pandas-dev/pandas'
 matplotlib = "pandas:plotting._matplotlib"
 
 [project.optional-dependencies]
-test = ['hypothesis>=6.46.1', 'pytest>=7.3.2', 'pytest-xdist>=2.2.0', 'pytest-asyncio>=0.17.0']
+test = ['hypothesis>=6.46.1', 'pytest>=7.3.2', 'pytest-xdist>=2.2.0']
 performance = ['bottleneck>=1.3.6', 'numba>=0.56.4', 'numexpr>=2.8.4']
 computation = ['scipy>=1.10.0', 'xarray>=2022.12.0']
 fss = ['fsspec>=2022.11.0']
@@ -110,7 +110,6 @@ all = ['beautifulsoup4>=4.11.2',
        'pyreadstat>=1.2.0',
        'pytest>=7.3.2',
        'pytest-xdist>=2.2.0',
-       'pytest-asyncio>=0.17.0',
        'python-calamine>=0.1.6',
        'pyxlsb>=1.0.10',
        'qtpy>=2.3.0',
@@ -153,7 +152,7 @@ setup = ['--vsenv'] # For Windows
 skip = "cp36-* cp37-* cp38-* pp* *_i686 *_ppc64le *_s390x *-musllinux_aarch64"
 build-verbosity = "3"
 environment = {LDFLAGS="-Wl,--strip-all"}
-test-requires = "hypothesis>=6.46.1 pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-asyncio>=0.17"
+test-requires = "hypothesis>=6.46.1 pytest>=7.3.2 pytest-xdist>=2.2.0"
 test-command = """
   PANDAS_CI='1' python -c 'import pandas as pd; \
   pd.test(extra_args=["-m not clipboard and not single_cpu and not slow and not network and not db", "-n 2", "--no-strict-data-files"]); \
@@ -519,7 +518,6 @@ markers = [
   "arm_slow: mark a test as slow for arm64 architecture",
   "arraymanager: mark a test to run with ArrayManager enabled",
 ]
-asyncio_mode = "strict"
 
 [tool.mypy]
 # Import discovery
diff --git a/requirements-dev.txt b/requirements-dev.txt
index a7c2237d1664d..490e170299030 100644
--- a/requirements-dev.txt
+++ b/requirements-dev.txt
@@ -9,7 +9,6 @@ meson-python==0.13.1
 pytest>=7.3.2
 pytest-cov
 pytest-xdist>=2.2.0
-pytest-asyncio>=0.17.0
 coverage
 python-dateutil
 numpy<2
diff --git a/scripts/tests/data/deps_expected_random.yaml b/scripts/tests/data/deps_expected_random.yaml
index 1ede20f5cc0d8..2c1f987e8101a 100644
--- a/scripts/tests/data/deps_expected_random.yaml
+++ b/scripts/tests/data/deps_expected_random.yaml
@@ -14,7 +14,6 @@ dependencies:
   - pytest-cov
   - pytest-xdist>=2.2.0
   - psutil
-  - pytest-asyncio>=0.17.0
   - boto3
 
   # required dependencies
diff --git a/scripts/tests/data/deps_minimum.toml b/scripts/tests/data/deps_minimum.toml
index 501ec4f061f17..9856d230c3de1 100644
--- a/scripts/tests/data/deps_minimum.toml
+++ b/scripts/tests/data/deps_minimum.toml
@@ -55,7 +55,7 @@ repository = 'https://github.com/pandas-dev/pandas'
 matplotlib = "pandas:plotting._matplotlib"
 
 [project.optional-dependencies]
-test = ['hypothesis>=6.34.2', 'pytest>=7.3.2', 'pytest-xdist>=2.2.0', 'pytest-asyncio>=0.17.0']
+test = ['hypothesis>=6.34.2', 'pytest>=7.3.2', 'pytest-xdist>=2.2.0']
 performance = ['bottleneck>=1.3.2', 'numba>=0.53.1', 'numexpr>=2.7.1']
 timezone = ['tzdata>=2022.1']
 computation = ['scipy>=1.7.1', 'xarray>=0.21.0']
@@ -102,7 +102,6 @@ all = ['beautifulsoup4>=5.9.3',
        'pyreadstat>=1.1.2',
        'pytest>=7.3.2',
        'pytest-xdist>=2.2.0',
-       'pytest-asyncio>=0.17.0',
        'python-calamine>=0.1.6',
        'pyxlsb>=1.0.8',
        'qtpy>=2.2.0',
@@ -142,7 +141,7 @@ parentdir_prefix = "pandas-"
 [tool.cibuildwheel]
 skip = "cp36-* cp37-* pp37-* *-manylinux_i686 *_ppc64le *_s390x *-musllinux*"
 build-verbosity = "3"
-test-requires = "hypothesis>=6.34.2 pytest>=7.3.2 pytest-xdist>=2.2.0 pytest-asyncio>=0.17"
+test-requires = "hypothesis>=6.34.2 pytest>=7.3.2 pytest-xdist>=2.2.0"
 test-command = "python {project}/ci/test_wheels.py"
 
 [tool.cibuildwheel.macos]
@@ -386,7 +385,6 @@ markers = [
   "arm_slow: mark a test as slow for arm64 architecture",
   "arraymanager: mark a test to run with ArrayManager enabled",
 ]
-asyncio_mode = "strict"
 
 [tool.mypy]
 # Import discovery
diff --git a/scripts/tests/data/deps_unmodified_random.yaml b/scripts/tests/data/deps_unmodified_random.yaml
index 14bedd1025bf8..f3772d9e20a1b 100644
--- a/scripts/tests/data/deps_unmodified_random.yaml
+++ b/scripts/tests/data/deps_unmodified_random.yaml
@@ -14,7 +14,6 @@ dependencies:
   - pytest-cov
   - pytest-xdist>=2.2.0
   - psutil
-  - pytest-asyncio>=0.17
   - boto3
 
   # required dependencies
diff --git a/tooling/debug/Dockerfile.pandas-debug b/tooling/debug/Dockerfile.pandas-debug
index 00e10a85d7ab9..d674e9c0ee1e0 100644
--- a/tooling/debug/Dockerfile.pandas-debug
+++ b/tooling/debug/Dockerfile.pandas-debug
@@ -23,7 +23,6 @@ RUN python3 -m pip install \
     meson \
     meson-python \
     pytest \
-    pytest-asyncio \
     python-dateutil \
     pytz \
     versioneer[toml]