diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 5b11490479088..2f46190ef5eb7 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -35,7 +35,7 @@ repos:
         exclude: ^pandas/_libs/src/(klib|headers)/
         args: [--quiet, '--extensions=c,h', '--headers=h', --recursive, '--filter=-readability/casting,-runtime/int,-build/include_subdir']
 -   repo: https://gitlab.com/pycqa/flake8
-    rev: 3.9.0
+    rev: 3.9.1
     hooks:
     -   id: flake8
         additional_dependencies:
@@ -75,7 +75,7 @@ repos:
     hooks:
     -   id: yesqa
         additional_dependencies:
-            - flake8==3.9.0
+            - flake8==3.9.1
             - flake8-comprehensions==3.1.0
             - flake8-bugbear==21.3.2
             - pandas-dev-flaker==0.2.0
diff --git a/environment.yml b/environment.yml
index 146bf6db08d8b..0d03ad8e0a46a 100644
--- a/environment.yml
+++ b/environment.yml
@@ -20,7 +20,7 @@ dependencies:
   # code checks
   - black=20.8b1
   - cpplint
-  - flake8=3.9.0
+  - flake8=3.9.1
   - flake8-bugbear=21.3.2  # used by flake8, find likely bugs
   - flake8-comprehensions=3.1.0  # used by flake8, linting of unnecessary comprehensions
   - isort>=5.2.1  # check that imports are in the right order
diff --git a/requirements-dev.txt b/requirements-dev.txt
index 33deeef9f1f82..ea7ca43742934 100644
--- a/requirements-dev.txt
+++ b/requirements-dev.txt
@@ -8,7 +8,7 @@ asv
 cython>=0.29.21
 black==20.8b1
 cpplint
-flake8==3.9.0
+flake8==3.9.1
 flake8-bugbear==21.3.2
 flake8-comprehensions==3.1.0
 isort>=5.2.1