diff --git a/pandas-stubs/core/frame.pyi b/pandas-stubs/core/frame.pyi
index 8ffe3bac2..a036bf176 100644
--- a/pandas-stubs/core/frame.pyi
+++ b/pandas-stubs/core/frame.pyi
@@ -157,6 +157,7 @@ class _LocIndexerFrame(_LocIndexer):
         self,
         idx: IndexType
         | MaskType
+        | Callable[[DataFrame], IndexType | MaskType | list[HashableT]]
         | list[HashableT]
         | tuple[
             IndexType | MaskType | list[HashableT] | Hashable,
@@ -167,14 +168,22 @@ class _LocIndexerFrame(_LocIndexer):
     def __getitem__(
         self,
         idx: tuple[
-            int | StrLike | tuple[Scalar, ...], int | StrLike | tuple[Scalar, ...]
+            int | StrLike | tuple[Scalar, ...] | Callable[[DataFrame], ScalarT],
+            int | StrLike | tuple[Scalar, ...],
         ],
     ) -> Scalar: ...
     @overload
     def __getitem__(
         self,
         idx: ScalarT
-        | tuple[IndexType | MaskType | _IndexSliceTuple, ScalarT | None]
+        | Callable[[DataFrame], ScalarT]
+        | tuple[
+            IndexType
+            | MaskType
+            | _IndexSliceTuple
+            | Callable[[DataFrame], ScalarT | list[HashableT] | IndexType | MaskType],
+            ScalarT | None,
+        ]
         | None,
     ) -> Series: ...
     @overload
diff --git a/tests/test_frame.py b/tests/test_frame.py
index 66716d79a..e31c05a21 100644
--- a/tests/test_frame.py
+++ b/tests/test_frame.py
@@ -22,6 +22,7 @@
     TypedDict,
     TypeVar,
     Union,
+    cast,
 )
 
 import numpy as np
@@ -2366,6 +2367,27 @@ def test_frame_dropna_subset() -> None:
     )
 
 
+def test_loc_callable() -> None:
+    # GH 256
+    df = pd.DataFrame({"x": [1, 2, 3], "y": [4, 5, 6]})
+
+    def select1(df: pd.DataFrame) -> pd.Series:
+        return df["x"] > 2.0
+
+    check(assert_type(df.loc[select1], pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.loc[select1, :], pd.DataFrame), pd.DataFrame)
+
+    def select2(df: pd.DataFrame) -> list[Hashable]:
+        return [i for i in df.index if cast(int, i) % 2 == 1]
+
+    check(assert_type(df.loc[select2, "x"], pd.Series), pd.Series)
+
+    def select3(df: pd.DataFrame) -> int:
+        return 1
+
+    check(assert_type(df.loc[select3, "x"], Scalar), np.integer)
+
+
 def test_npint_loc_indexer() -> None:
     # GH 508