diff --git a/src/diffusers/pipelines/ltx/pipeline_ltx_image2video.py b/src/diffusers/pipelines/ltx/pipeline_ltx_image2video.py
index 6c4214fe1b26..0f640dc33546 100644
--- a/src/diffusers/pipelines/ltx/pipeline_ltx_image2video.py
+++ b/src/diffusers/pipelines/ltx/pipeline_ltx_image2video.py
@@ -487,19 +487,21 @@ def prepare_latents(
     ) -> torch.Tensor:
         height = height // self.vae_spatial_compression_ratio
         width = width // self.vae_spatial_compression_ratio
-        num_frames = (
-            (num_frames - 1) // self.vae_temporal_compression_ratio + 1 if latents is None else latents.size(2)
-        )
+        num_frames = (num_frames - 1) // self.vae_temporal_compression_ratio + 1
 
         shape = (batch_size, num_channels_latents, num_frames, height, width)
         mask_shape = (batch_size, 1, num_frames, height, width)
 
         if latents is not None:
-            conditioning_mask = latents.new_zeros(shape)
+            conditioning_mask = latents.new_zeros(mask_shape)
             conditioning_mask[:, :, 0] = 1.0
             conditioning_mask = self._pack_latents(
                 conditioning_mask, self.transformer_spatial_patch_size, self.transformer_temporal_patch_size
-            )
+            ).squeeze(-1)
+            if latents.ndim != 3 or latents.shape[:2] != conditioning_mask.shape:
+                raise ValueError(
+                    f"Provided `latents` tensor has shape {latents.shape}, but the expected shape is {conditioning_mask.shape + (num_channels_latents,)}."
+                )
             return latents.to(device=device, dtype=dtype), conditioning_mask
 
         if isinstance(generator, list):