diff --git a/aws_lambda_powertools/event_handler/middlewares/openapi_validation.py b/aws_lambda_powertools/event_handler/middlewares/openapi_validation.py
index 54c48189282..25ac97ddf89 100644
--- a/aws_lambda_powertools/event_handler/middlewares/openapi_validation.py
+++ b/aws_lambda_powertools/event_handler/middlewares/openapi_validation.py
@@ -388,7 +388,7 @@ def _normalize_multi_query_string_with_param(query_string: Optional[Dict[str, st
             try:
                 # if the target parameter is a scalar, we keep the first value of the query string
                 # regardless if there are more in the payload
-                query_string[param.name] = query_string[param.name][0]
+                query_string[param.alias] = query_string[param.alias][0]
             except KeyError:
                 pass
     return query_string
diff --git a/tests/functional/event_handler/test_openapi_validation_middleware.py b/tests/functional/event_handler/test_openapi_validation_middleware.py
index 07e2a34ac42..be3a13dd656 100644
--- a/tests/functional/event_handler/test_openapi_validation_middleware.py
+++ b/tests/functional/event_handler/test_openapi_validation_middleware.py
@@ -2,7 +2,7 @@
 from dataclasses import dataclass
 from enum import Enum
 from pathlib import PurePath
-from typing import List, Tuple
+from typing import List, Optional, Tuple
 
 import pytest
 from pydantic import BaseModel
@@ -15,9 +15,11 @@
     Response,
     VPCLatticeResolver,
     VPCLatticeV2Resolver,
+    content_types,
 )
 from aws_lambda_powertools.event_handler.openapi.params import Body, Header, Query
 from aws_lambda_powertools.shared.types import Annotated
+from aws_lambda_powertools.utilities.data_classes import APIGatewayProxyEvent
 from tests.functional.utils import load_event
 
 LOAD_GW_EVENT = load_event("apiGatewayProxyEvent.json")
@@ -1018,3 +1020,23 @@ def handler3():
     # IF expected_error_text is provided, THEN check for its presence in the response body
     if expected_error_text:
         assert any(text in result["body"] for text in expected_error_text)
+
+
+def test_validation_with_alias():
+    # GIVEN a Http API V2 proxy type event
+    app = APIGatewayRestResolver(enable_validation=True)
+    event = load_event("apiGatewayProxyEvent.json")
+
+    class FunkyTown(BaseModel):
+        parameter: str
+
+    @app.get("/my/path")
+    def my_path(
+        parameter: Annotated[Optional[str], Query(alias="parameter1")] = None,
+    ) -> Response[FunkyTown]:
+        assert isinstance(app.current_event, APIGatewayProxyEvent)
+        assert parameter == "value1"
+        return Response(200, content_types.APPLICATION_JSON, FunkyTown(parameter=parameter))
+
+    result = app(event, {})
+    assert result["statusCode"] == 200